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To detect the quinolone resistance genes of Staphylococcus aureus and analyze their relationship with efflux pump genes in quinolone-resistant strains. Antibiotic sensitivity of S. aureus strains isolated from clinical specimens was determined using the micro-broth dilution method. The expression of NorA and Qnr was analyzed using real-time PCR. The mutation status of gyrA was investigated by PCR and DNA sequencing. In total, 119 strains of S. aureus were collected. The oxacillin minimum inhibitory concentration (MIC) was high (≥ 8 μg/mL) in 5 strains. Resistance to moxifloxacin and ciprofloxacin, with the MICs of ≥ 2 μg/mL, was seen in 7 strains. The expression of QnrS and NorA was detected in 5 and 12 strains, respectively. All of the strains had wild-type gyrA. Mutation at Ser81 or at both positions was found in only one strain. No relationship was found between QnrS and NorA, but their high expression in quinolone-resistant strains could contribute to the resistance to moxifloxacin. QnrS and NorA could be developed as targets in these strains for quinolone therapy.
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